Alanine aminotransferase (ALT), also known as serum glutamic pyruvic transaminase (SGPT) or alanine transaminase (ALAT) is a transaminase enzyme. ALT can be found in serum and different body tissues and is mainly found in liver. It catalyzes the reaction: α-ketoglutarate + alanine ⇌ glutamate + pyruvate. It is commonly used as a clinical test for liver health. In Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric/Fluorometric Assay Kit, ALT catalyzes the transfer of an amino group from alanine to ⍺-ketoglutarate and produce pyruvate and glutamate. The pyruvate can be detected in a reaction that concomitantly transfrom a nearly colorless probe to both color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). This kit offers a reliable and sensitive way for high throughput activity assay of ALT with a detection limit of 0.05 mU per well.
Features & Properties
Features
Simple procedure; Fast and convenient;
Sensitive assays for measuring ALT activity in various biological samples and the kit is also suitable for high throughput analyses.
