Annexin V-FITC Apoptosis Kit Plus

Soon after the apoptosis is activated, most cell types transfer the membrane phospholipid phosphatidylserine (PS) from the plasma membrane inner face to the cell surface. Detection of the cell-surface PS can be easily done by staining with a fluorescent conjugate of protein Annexin V which has a robust natural affinity for PS. The one-step staining process needs just 10 minute. This assay can be directly carried out on live cells without fixation. 

The Annexin V-FITC Apoptosis Detection Kit Plus includes annexin V-FITC, SYTOX green dye, and binding buffer. The SYTOX green dye is impermeant to live cells and apoptotic cells, but stains necrotic cells with intense green fluorescence by binding to cellular nucleic acids. Following the staining the cell population with annexin V-FITC and SYROX Green dye in the given binding buffer, apoptotic cells exhibit green fluorescence, dead cells exhibits a higher level of green florescence and lives cells exhibits little or no fluorescence. 

Those cell populations can be differentiated with flow cytomery at excitation of 488 nm and emission of 530 nm.Both annexin V-FITC and SYTOX Green dye emit green fluorescence that can be detected in the FL1 channel, freeing the other channels for the addition of other probes in multi-color labeling experiments