Internucleosomal DNA fragmentation is a sign of apoptosis in mammalian cells. The BrdU In Situ DNA Fragmentation Assay Kit provides a simple and convenient way for detection of DNA fragmentation in biological samples based on fluorescence microscopy or flow cytometry method. The kit provides complete components including positive and negative control cells. The assay uses Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more easily incorporated into DNA strand breaks than other larger ligands such as digoxigenin, fluorescein and biotin. The greater incorporation generates brighter signal and the Br-dUTP sites can be identified by a fluorescein labeled anti-BrdU monoclonal antibody.
Features & Properties
Features
Fast and convenient
Simple procedure; takes 1 hour
The assay is sensitive and stable.
The incorporation of BrdU in the procedure provides greater sensitivity than biotinylated or digoxigenylated methods.