Ascorbic Acid Colorimetric/Fluorometric Assay Kit
Ascorbic Acid is a reductone and plays important roles in many biological processes. Ascorbic Acid is present in a wide variety of biological specimens and foods. Ascorbic Acid is a potent anti-oxidant, anti-viral and anti-inflammatory agent, and acts as an immune stimulant. Measurement of ascorbic acid content in different samples is important.
The Ascorbic Acid Colorimetric/Fluorometric Assay Kit provides a sensitive, fast and convenient way for detection of ascorbic acid levels in various samples based on colorimetric and fluorometric method. In the assay, the Catalyst oxidizes ascorbic acid to generate a product that interacts with the ascorbic acid probe, generating fluorescence (Ex/Em = 535/587 nm) and color (spectrophotometry at λ = 570 nm). The kit can detect 0.01-10 nmol ascorbic acid in various samples.
| Features | Fast and convenient; Simple procedure; Takes approx. 5 minutes for the reaction to complete. |
| Shipping | Gel pack. |
| Storage Conditions | Store at -20°C. |
| Kit components | Ascorbic Acid Assay Buffer, Ascorbic Acid Probe (lyophilized), Dimethylsulfoxide (DMSO, Anhydrous), Catalyst, Ascorbic Acid Enzyme Mix (lyophilized), Ascorbic Acid Standard (20 µmole) |