Caspase-12 Fluorometric Assay Kit

Caspases (Cysteine-dependent aspartate-directed proteases) are a family of cysteine proteases that play important roles in apoptosis, inflammation and necrosis. Sequential activation of caspases plays an important role in cell apoptosis. Caspase-12 is an inflammatory caspase that is involved in processing and activating inflammatory cytokines such as interleukin 1 and interleukin 18. Inhibition of caspase-12 leads to a stronger inflammatory reaction to bacterial pathogens.

Caspase-12 Fluorometric Assay Kit provides a highly sensitive, simple and convenient way for detecting the ATAD-dependent caspase activity based on detection of cleavage of substrate ATAD-AFC. ATAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin) emits blue light (λmax = 400 nm); while cleavage of ATAD-AFC by caspase-12 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm) that can be quantified by using a fluorecence microtiter plate reader or a fluorometer. Comparison of the fluorescence from an apoptotic sample with an uninduced control determines the fold increase in caspase-12 activity.