Caspase-5 Colorimetric Assay Kit
Caspase-5 belongs to the Caspase-family of cysteine proteases. Caspase-5 is an enzyme that proteolytically cleaves other proteins at an aspartic acid residue. Caspase-5 exists in cells as an inactive proenzyme and is matured by proteolysis. The active Caspase-5 is a heterotetramer containing two large and two small subunits. The function of Caspase-5 is believed to be an inflammatory Caspase, together with Caspase-1, Caspase-4, and has a role in the immune system.
The Caspase-5 Colorimetric Assay Kit provides a fast and convenient means for assaying the activity of Caspase-5 and other related caspases. These caspases can recognize the sequence WEHD. The assay is according to spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate WEHD-pNA. Using a spectrophotometer or a microtiter plate reader at 400 or 405 nm can quantify the pNA light emission. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-5 activity.
| Features | Fast and convenient; Simple one-step procedure; Takes only 1-2 hours; Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-5 activity. |
| Shipping | Gel pack. |
| Storage Conditions | Store at -20°C. |
| Kit components | Cell Lysis Buffer, 2X Reaction Buffer, WEHD-pNA (4 mM), DTT (1 M), Dilution Buffer |