Caspase Colorimetric Substrate Set II Plus
Caspases (Cysteine-dependent aspartate-directed proteases) are a family of cysteine proteases that play important roles in apoptosis, inflammation and necrosis. Sequential activation of caspases plays a critical role in cell apoptosis. Caspase-1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 are members of caspases family. Caspase-1, 4 and 5 are involved in T-cell maturation. Caspase-2, 8, 9 and 10 are initiator caspases that cleave inactive pro-forms of effector caspases into active caspases. Caspase-3, 6 and 7 are effector caspases that cleave other protein substrates within the cell to trigger the apoptotic process.
Caspase Colorimetric Substrate Set II Plus is composed of ready-to-use pNA-labeled substrates for caspase-1, 2, 3, 4, 5, 6, 8, 9 and 10 of caspase family proteases. The kit is used to detect activities of members of caspase family proteases. All substrates are provided in liquid form.
| Features | Contains ready-to-use caspase-1/-2/-3/-4/-5/-6/-8/-9/-10 pNA-labeled substrates and all buffers for performing caspase assay. |
| Shipping | Gel pack. |
| Storage Conditions | Store at -20°C. |
| Kit components | Caspase-1 Substrate, Ac-YVAD-pNA, Caspase-2 Substrate, Ac-VDVAD-pNA, Caspase-3 Substrate, Ac-DEVD-pNA , Caspase-4 Substrate, Ac-LEVD-pNA, Caspase-5 Substrate, Ac-WEHD-pNA, Caspase-6 Substrate, Ac-VEID-pNA, Caspase-8 Substrate, Ac-IETD-pNA, Caspase-9 Substrate, Ac-LEHD-pNA, Caspase-10 Substrate, Ac-AEVD-pNA, Cell Lysis Buffer, Dilution Buffer, 2X Reaction Buffer, DTT (1 M) |