CETP Activity Fluorometric Assay Kit

Cholesteryl ester transfer protein (CETP) is a plasma protein that transfers a cholesteryl ester from high-density lipoproteins (HDL) to low-density lipoproteins (LDL) or very-low-density lipoproteins (VLDL) in exchange for a triglyceride, and vice versa. HDL plays important roles in lipid metabolism and cardiovascular health. HDL transports cholesterol to steroidogenic tissues for steroid synthesis or to the liver for excretion. HDL is also involved in the reverse cholesterol transport pathway, protecting against atherosclerosis by removing cholesterol from lipid-filled macrophages. CETP is known as a target to increase HDL.

The CETP Activity Fluorometric Assay Kit provides a simple and convenient way for detection of CETP activity in various samples based on fluorometric method. The assay utilizes a self-quenched fluorescent neutral lipid that can be detected when transferred to an acceptor molecule. The fluorometric intensity is directly proportional to the amount of neutral lipid transfer. CETP inhibitor Torcetrapib is provided for assay validation and rabbit serum is included as a positive control. In addition to detecting CETP activity in serum, the kit is also suited for detection of recombinant protein activity.