cGMP Direct Immunoassay Kit (Colorimetric)

Cyclic guanosine monophosphate (cGMP) is a second messenger that plays an important role in intracellular signal transduction through the activation of protein kinases and is synthesized from GTP by guanylate cyclase.

The cGMP Direct Immunoassay Kit (Colorimetric) provides a highly sensitive and convenient way for detection of cGMP level in various biological samples based on direct competitive immunoassay and colorimetric method. The recombinant Protein G coated 96-well plate is utilized to efficiently anchor cGMP polyclonal antibody on the plate. Then, cGMP-HRP competes with cGMP from samples for binding to the cGMP polyclonal antibody on the plate. After incubation and washing, the amount of cGMP-HRP bound to the plate can be easily determined by reading HRP activity at OD450 nm. The intensity of absorbance is inversely proportional to the concentration of cGMP in samples. In addition, the assay provides a new acetylation method that significantly improves detection sensitivity. The assay can detect 0.04 -10 pmol/well (0.008 - 2 µM) cGMP levels.