Cytochrome Oxidase Activity Colorimetric Assay Kit

The cytochrome c oxidase (complex IV) is a transmembrane protein complex exists in bacteria and the mitochondrion of eukaryotes. Cytochrome c Oxidase is the last enzyme in the respiratory electron transport chain located in the bacterial or mitochondrial membrane. Cytochrome c Oxidase receives an electron from each of four cytochrome c molecules, and transfers it to O₂, converting O₂ to two molecules of H₂O. In the process, it binds four protons from the inner aqueous phase to make H₂O, and translocates four protons across the membrane to establish a proton electrochemical potential that is used to synthesize ATP by the ATP synthase. Cytochrome c Oxidase provides energy to the cell by coupling electron transport through the respiratory electron transport chain with oxidative phosphorylation.

 Cytochrome Oxidase Activity Colorimetric Assay Kit is fast, simple and high-throughput adaptable. This assay kit can be used for cells/tissue extracts containing mitochondria or purified mitochondria. The activity of cytochrome oxidase is detected colorimetrically by following the oxidation of decreased Cytochrome c as an absorbance decrease at 550 nm.