Glucose Colorimetric/Fluorometric Assay Kit

Glucose (C₆H₁₂O₆) is an important fuel source to produce energy molecule ATP. Glucose level is a key diagnostic parameter for many metabolic disorders. Measurement of glucose is very important in both drug discovery and research processes.

The Glucose Colorimetric/Fluorometric Assay Kit provides a sensitive, simple and convenient way for detection of glucose in various biological samples (serum, plasma, body fluid, growth medium, food, etc.) based on colorimetric and fluorometric method. In the assay, Glucose Enzyme Mix specifically oxidizes glucose to yield a product which reacts with a dye to produce fluorescence (Ex/Em = 535/587 nm) and color (λ = 570 nm). The color and fluorescence generated is proportionally to the amount of glucose. The assay is suited for high throughput screening and is also suited for monitoring glucose feeding in protein expression processes and glucose level during fermentation. The kit can detect 1-10000 µM glucose samples.