Glutathione (GSH) is a low-molecular-weight thiol that play a vital role in protecting cell from oxidative stress in mammalian cells. The Glutathione Colorimetric Assay Kit provides an easy and colorimetic method for analyzing totoal glutathione or the reduced from of glutathione by a microtiter plate reader. The assay utilizes glutathione recycling system by DTNB and glutathione reductase. The yellow color 2-nitro-5-thiobenzoic acid that generate by the reaction of DTNB and GSH. Thus the GSH concentration can be measured at 412 nm absorbance. The generated GSSG can be reduced back to GSH by glutathione reductase, and GSH reacts with DTNB again to produce more 2-nitro-5-thiobenzoic acid. As a result, the recycling system significantly improves the sensitivity of total GSH detection. The 5-Sulfosalicylic acid (SSA) in the kit removes proteins from samples to protect the GSH oxidation and γ-glutamyl transpeptidase reaction. The kit can quantify glutathione from 1-100 ng/well in a 200 μL reaction. Increase the reaction time to generate stronger signal for detecting lower glutathione concentrations, such as in blood samples. The kit can also specifically detect the reduced form of glutathione (GSH) by omitting the glutathione reductase from the reaction mixture. The sensitivity for detecting the reduced form of glutathione (without recycling system) is 100 times lower than detecting the total glutahione.
Features & Properties
Features
Simple procedure; Takes only approx. 2-3 hours;
Fast and convenient.
