Lactate Colorimetric Assay Kit II

Lactate is chiral: L(+)-Lactate and D(-)-Lactate. L(+)-Lactate exists in blood and is constantly produced from pyruvate by lactate dehydrogenase (LDH) in human intermediary metabolism. D(-)-Lactate is present only at about 1-5% of the concentration of L(+)-Lactate. Abnormal high concentration of lactate is related to diseases such as lactate acidosis and diabetes.

The Lactate Colorimetric Assay Kit II provides a sensitive, simple and convenient way for detection of lactate in various biological samples (plasma, serum, culture and fermentation media, cells, etc.) based on colorimetric method. In the assay, lactate is oxidized by lactate dehydrogenase to product a product which interacts with a probe to yield a color (λmax = 450 nm). The assay can be performed without pretreatment or purification of samples. The kit can detect 0.02 mM-10 mM lactate in various samples.