Lactose Colorimetric/Fluorometric Assay Kit

Lactose (C₁₂H₂₂O₁₁) is a disaccharide sugar consisting of one galactose and one glucose. Lactose makes up around 2-8% of milk. Some people, particularly infants, lack lactase to digest lactose leading to lactose accumulation in blood (Galactosemia), which then causes renal failure, enlarged liver, cataracts and brain damage.

The Lactose Colorimetric/Fluorometric Assay Kit provides a sensitive, fast and convenient way for detection of lactose levels in various biological samples (plasma, serum, other body fluids, growth media, food, etc.) based on colorimetric and fluorometric method. In the assay, lactose is hydrolyzed to glucose and galactose, which is then oxidized producing fluorescence (Ex/Em 535/587 nm) and color (O.D 570 nm). Free galactose can be corrected by a background control in the absence of lactase. The assay is performed without pretreatment of samples. The kit is suited for a high throughput assay.