Malate Dehydrogenase (MDH) is an enzyme that reversibly catalyzes the oxidation of L-malate to oxaloacetate in the presence of NAD+. MDH has 2 isoforms: MDH1 and MDH2. MDH1 exists in cytosolic and participates in the malate-aspartate shuttle, which transports malate into mitochondria for ATP production. However, MDH2 is present in mitochondria and is involved in the citric acid cycle. In severe liver damage such as hepatocellular carcinoma, MDH activity in serum is abnormal, which can be used as a diagnostic tool. In addition, MDH activity is increased in some neurodegenerative diseases such as Alzheimer’s disease. The Malate Dehydrogenase Activity Colorimetric Assay Kit provides a highly sensitive, simple, fast and convenient way for detection of MDH activity in various biological samples based on colorimetric method. In the assay, MDH reacts with malate to form an intermediate, which reacts with MDH Developer to yield a colored product with strong absorbance at 450 nm. The kit can detect less than 0.5 mU MDH activity in various sample types.
Features & Properties
Features
Simple, rapid and convenient;
The assay can detect malate dehydrogenase (MDH) activity less than 0.5 mU in various sample types.
MDH Assay Buffer,
MDH Substrate (Lyophilized),
MDH Enzyme Mix (Lyophilized),
MDH Developer (Lyophilized),
NADH Standard (Lyophilized),
MDH Positive Control (Lyophilized)
