Mitochondria/Cytosol Fractionation Kit
Catalog No.
K2103
Isolates mitochondria/cytosol w/o ultracentrifugation
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The Mitochondria/Cytosol Fractionation Kit utilize a special formulation of reagents to isolate enriched mitochondrial fraction from cytosolic fraction of both apoptotic and nonapoptotic mammalian cells. The enriched mitochondrial and cytosolic fraction can be used to detect translocation of factors in apoptosis and signaling pathways with Western blotting, ELISA and other assays etc. The protocol is simple to perform without unltracentrifugations and toxic chemicals.
| Features | Simple procedure; Takes only 3-4 hours; Fast and convenient; The fractionation procedure is simple and straightforward. No ultracentrifugation is required. No toxic chemicals are involved. |
| Shipping | Gel pack. |
| Storage Conditions | Store at -20°C. |
Store at -20°C.
| Kit components | Mitochondria Extraction Buffer, 5X Cytosol Extraction Buffer, DTT (1 M), Protease Inhibitor Cocktail |