Nitric Oxide Fluorometric Assay Kit
Catalog No.
K2099
Detects total nitrate/nitrite
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Nitric oxide is essential in neurotransmission, vascular regulation, immune response and apoptosis. Because Nitric oxide is rapidly converted into nitrite and nitrate, the NO production can be quantitatively determined by the total concentration of nitrite and nitrate. This kit is a simple and accurate two-step measurement of total nitrate/nitrite concentration. Nitrate reductase converts nitrate into nitrite first, and then nitrite reacts with the fluorescent probe DAN (2, 3 diaminonaphthalene). NaOH enhances the fluorescent yield. The total nitric oxide production is proportional to the fluorescent intensity. The kit has been tested with culture media, plasma, and tissue homogenates.
| Features | Simple procedure; Takes only 2-3 hours; Fast and convenient. |
| Shipping | Gel pack. |
| Storage Conditions | Store at -20°C. |
Store at -20°C.
| Kit components | Assay Buffer, Enzyme Cofactor, Enhancer, Nitrate Reductase, Nitrate Standard, Nitrite Standard, DAN Probe, Sodium Hydroxide, Microtiter plate, Plate Cover |