3T3-msCD40L Cell Lines
Catalog No.
BC1002
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3T3-CD40L is a mouse fibroblast cell line that secretes mouse CD40L. 3T3-CD40L cells can be co-cultured with B cells to aid B cell expansion [1].
Reference：
[1] J. Huang, N. A. Doria-Rose, N. S. Longo, L. Laub, C.-L. Lin, E. Turk, B. H. Kang, S. A. Migueles, R. T. Bailer, J. R. Mascola, M. Connors, Isolation of Human Monoclonal Antibodies from Peripheral Blood B Cells. Nat. Protoc. 8, 1907-1915 (2013).
| Alternative name | 3T3-msCD40L, 3T3-CD40L |
| Cell Type | Mouse 3T3 cell line expression CD40L |
| Accession ID | CVCL_1H10 |
| Organism | Mouse |
| Biosafety Level | 2 |
| Subculturing | Adherent Cells, like fibroblast cells |
| Maintenance Tips | 3T3-CD40L cells should be irradiated in advance so that the cells secrete mouse CD40L for B cell activation and die off. Please see the reference for more details. |
| Propagation Medium | DMEM with 10% FBS, 1% L-glutamine, and 0.1% gentamicin. |
| Storage | -150°C~-196°C (liquid-nitrogen). |
| Shipping Condition | Ship with dry ice (-80°C). |
| Note | For laboratory research only. Not for clinical applications. |
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