Annexin V-HF647/7-AAD Apoptosis Kit
Annexin V is a cellular protein, which plays important roles in the inhibition of the activity of phospholipase A1 and blood coagulation by competing for phosphatidylserine (PS) binding sites with prothrombin. Annexin V has a high affinity to PS and is used as a probe to detect cells that have expressed PS on the cell surface. After initiating apoptosis, cells translocate phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface soon, which can be easily detected by Annexin V.
Annexin V-HF647/7-AAD Apoptosis Kit uses a fluorescent conjugate of Annexin V that can easily detect PS on the cell surface after initiating apoptosis. The one-step staining procedure needs only 10-20 minutes. The result can be analyzed by fluorescence microscopy or by flow cytometry. The Annexin V-HF647/PI Apoptosis Kit can differentiate apoptosis vs necrosis when performing both Annexin V-HF647 and 7-AAD staining.
图1：本试剂盒检测细胞凋亡验证图
| Components |
K2258-20 Assays |
K2258-50 Assays |
K2258-100 Assays |
|
Annexin V-HF647 |
100 μL |
250 μL |
500 μL |
|
10X Binding Buffer |
2.8 mL |
5.5 mL |
11 mL |
|
7-AAD |
100 μL |
250 μL |
500 μL |
|
Store the kit at 2-8°C stable for 6 months. Annexin V-HF647 and 7-AAD should be stored away from light. Do not freeze Annexin V-HF647. |
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| Features | Fast and convenient. Simple one step staining procedure in 10-20 minutes. Kit can differentiate apoptosis vs necrosis when performing both Annexin V-HF647 and 7-AAD staining. |