Cy5-UTP
Catalog No.
B8333
used as a substrate for T7 RNA polymerase and generate labeled probes through in vitro transcription
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Cy5-UTP (Cyanine 5-UTP) can replace UTP as a substrate for T7 RNA polymerase and generate labeled probes through in vitro transcription. The resulting assembled mRNA will emit orange fluorescence with the involvement of Cy5-UTPs. In addition, Cy5-labeled mRNA is readily visible under ultraviolet light without further staining after gel electrophoresis.
Probes generated by these methods are suitable for various application, such as FISH, multicolor fluorescence analysis, specifically dual-color expression arrays in conjunction with Cy5-UTP.
Excitation/Emission (nm): 650/665 nm
- 1. Yan Cao, Zongxing He, et al. "Helper-Polymer Based Five-Element Nanoparticles (FNPs) for Lung-Specific mRNA Delivery with Long-Term Stability after Lyophilization." Nano Lett. 2022 Aug 24;22(16):6580-6589. PMID:35969167
- 2. Shelby L Brown, Dana J Garrison, et al. "Phase separation of a plant virus movement protein and cellular factors support virus-host interactions." PLoS Pathog. PMID: 34543360
| Physical Appearance | Solution |
| Storage | Store at -20°C or below |
| M.Wt | 1178.01(free acid) |
| Formula | C45H58N5O22P3S2(free acid) |
| Synonyms | 5-(3-Aminoallyl)-uridine-5'-triphosphate;labeled with Cy5, Triethylammonium salt, Aminoallyl-UTP-Cy5, Cyanine 5-AA-UTP, Cyanine 5-Aminoallyluridine-5'-Triphosphate, Cyanine 5-UTP |
| Solubility | Soluble in H2O |
| Chemical Name | 2-((1E,3E,5E)-5-(1-(6-(((E)-3-(1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(((hydroxy((hydroxy(phosphonooxy)phosphoryl)oxy)phosphoryl)oxy)methyl)tetrahydrofuran-2-yl)-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)allyl)amino)-6-oxohexyl)-3,3-dimethyl-5-sulfoindolin-2-y |
| SDF | Download SDF |
| Canonical SMILES | O=S(C1=CC2=C(N(CCCCCC(NC/C=C/C3=CN([C@H]4[C@H](O)[C@H](O)[C@@H](COP(OP(O)(OP(O)(O)=O)=O)(O)=O)O4)C(NC3=O)=O)=O)/C(C2(C)C)=C/C=C/C=C/C(C5(C)C)=[N+](CC)C6=C5C=C(S([O-])(=O)=O)C=C6)C=C1)(O)=O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
- View current batch:
-
Purity = 95.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
