DAPI (hydrochloride)
DAPI (hydrochloride) is a fluorescent probe which serves as a DNA-specific probe for flow cytometry, chromosome staining, as well as DNA visualization and quantitation in histochemistry and biochemistry. DAPI forms a fluorescent complex by attaching to the minor grove of consecutive (3 ~ 4 base pairs) A-T rich sequences of DNA. Although DAPI is capable of binding to other sequences of double stranded DNA, or double stranded RNA, these complexes are much less fluorescent. DAPI can be used alone for cell cycle studies of drug effects, or in combination with another fluorochrome for multi-parameter cell analysis. One of the most popular stains combines DAPI with sulforhodamine (SR 101) for simultaneous measurement of DNA and protein content in cells. DAPI can be used on either fixed or live cells, but its low permeability in live cells suggests that higher concentrations should be used.
References:
1. Kapuscinski J. DAPI: a DNA-specific fluorescent probe. Biotechnic & Histochemistry, 1995, 70(5): 220-233.
2. Zink D, Sadoni N, Stelzer E. Visualizing chromatin and chromosomes in living cells. Methods, 2003, 29(1): 42-50.
- 1. ZHOU Qiyue, HONG Gaoying, et al. "Characterization and biocompatibility analysis of different silanes coupling c (RGDfK) cyclic peptide on titanium surfaces." J Prev Treat Stomatol Dis, 2022, 30(6): 398⁃405.
- 2. Liang Y, Wang J. "Sorafenib-Induced Exosome Secretion Promotes Che-motherapy Resistance in Hepatoma Cell Line." Japanese J Gstro Hepato. 2021; V6(12): 1-7.
| Storage | Store at -20°C |
| M.Wt | 350.25 |
| Cas No. | 28718-90-3 |
| Formula | C16H17Cl2N5 |
| Solubility | insoluble in EtOH; ≥10 mg/mL in H2O; ≥53.3 mg/mL in DMSO |
| Chemical Name | 2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide dihydrochloride |
| SDF | Download SDF |
| Canonical SMILES | NC(C(C=C1)=CC=C1C2=CC3=CC=C(C(N)=N)C=C3N2)=N.Cl.Cl |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Chemical structure
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