ddhCTP
Viperin, a member of the radical S-adenosyl-l-methionine (SAM) superfamily of enzymes, is an interferon-inducible protein implicated in the inhibition of replication of a broad range of RNA and DNA viruses, including dengue virus, West Nile virus, hepatitis C virus, influenza A virus, rabies virus and HIV. Viperin catalyses the conversion of cytidine triphosphate (CTP) to 3ʹ-deoxy-3′,4ʹ-didehydro-CTP (ddhCTP), a previously undescribed biologically relevant molecule, via a SAMdependent radical mechanism [1].
ddhCTP, can be produced in mammalian cytosol, is a chain terminator for the RNA-dependent RNA polymerases from multiple members of the Flavivirus genus, and directly inhibits replication of Zika virus in vivo[1].
Reference:
[1] Anthony S. Gizzi, Tyler L. Grove, Jamie J. Arnold, Joyce Jose, Rohit K. Jangra, Scott J. Garforth, Quan Du, Sean M. Cahill, Natalya G. Dulyaninova, James D. Love, Kartik Chandran, Anne R. Bresnick, Craig E. Cameron, Steven C. Almo. A naturally occurring antiviral ribonucleotide encoded by the human genome. Nature, 2018; DOI: 10.1038/s41586-018-0238-4.
| Physical Appearance | Solution |
| Storage | Store at -20°C or below |
| M.Wt | 465.14 |
| Formula | C9H14N3O13P3 |
| Synonyms | 3ʹ-deoxy-3′,4ʹ-didehydro-CTP |
| Solubility | Soluble in H2O |
| Chemical Name | (5-(4-amino-2-oxopyrimidin-1(2H)-yl)-4-hydroxy-4,5-dihydrofuran-2-yl)methyl tetrahydrogen triphosphate |
| SDF | Download SDF |
| Canonical SMILES | OC1C=C(COP(OP(OP(O)(O)=O)(O)=O)(O)=O)OC1N2C(N=C(N)C=C2)=O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment [1]: | |
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Cell lines |
Mammalian HEK293T cells. |
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Preparation method |
This compound can be soluble in H2O. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months. |
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Reacting condition |
0~72 h, 0, 1, 10, 100 and 300 μM |
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Applications |
ddhCTP inhibits replication of flavivirus (dengue virus, West Nile Virus and Zika virus) RNA-dependent RNA polymerase in HEK293T cells. |
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References: [1] Anthony S. Gizzi, Tyler L. Grove, Jamie J. Arnold, Joyce Jose, Rohit K. Jangra, Scott J. Garforth, Quan Du, Sean M. Cahill, Natalya G. Dulyaninova, James D. Love, Kartik Chandran, Anne R. Bresnick, Craig E. Cameron, Steven C. Almo. A naturally occurring antiviral ribonucleotide encoded by the human genome. Nature, 2018; DOI: 10.1038/s41586-018-0238-4. |
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Quality Control & MSDS
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Chemical structure
Related Biological Data
Related Biological Data
