Digoxigenin-11-ddUTP
Digoxigenin-11-ddUTP can be used as a substrate for: Terminal Transferase, DNA polymerase I (holoenzyme and Klenow fragment), T4 and T7 DNA polymerase or Taq DNA polymerase and reverse transcriptase (e.g., Transcriptor). It is preferentially used for 3'-end labeling of oligonucleotides with Terminal Transferase, recombinant. The DIG-labeled oligonucleotide can be used as a hybridization probe for: DNA and RNA transferring, Colony and plaque screening, in situ hybridization and so on. The resulting Digoxigenin-labeled oligonucleotide probes are subsequently detected using Digoxigenin-antibodies conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP) or a fluorescent dye. Optimal substrate properties and thus labeling efficiency is ensured by an 11-atom linker attached to the C5 position of uridine.
Recommended molar ratio of Digoxigenin-11-ddUTP to free 3'-OH groups: 10:1.
In addition to common hybridization techniques, DIG labeled oligonucleotides are specifically useful for screening expression libraries for sequence specific DNA-binding proteins such as transcription factors.
Please note: The optimal final concentration and molar ratio of Digoxigenin-11-ddUTP to free 3'-OH groups may depending on the application and assay conditions. For optimal incorporation rates an individual optimization of the Digoxigenin-11-ddUTP concentration and molar ratio is recommended.
References:
[1] Schmitzet al. (1991) Nonradioactive labeling of oligonucleotides in vitro with the hapten digoxigenin by tailing with terminal transferase. Anal Biochem199:222.
| Physical Appearance | Solution |
| Storage | Store at -20°C or below |
| M.Wt | 1048.90 (free acid) |
| Formula | C43H63N4O20P3 (free acid) |
| Synonyms | Digoxigenin-3-O-methylcarbonyl-ε-aminocaproyl-[5-(3-aminopropargyl) -2',3'-dideoxyuridine-5'-triphosphate], Triethylammonium salt, Dig-11-ddUTP |
| Solubility | Soluble in sterile water |
| Chemical Name | ((2S,5R)-5-(5-(3-(6-(2-(((3S,5R,8R,9S,10S,12R,13S,14S,17R)-12,14-dihydroxy-10,13-dimethyl-17-(5-oxo-2,5-dihydrofuran-3-yl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-yl)oxy)acetamido)hexanamido)prop-1-yn-1-yl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetra |
| SDF | Download SDF |
| Canonical SMILES | O=C1OCC([C@H]2CC[C@@]3(O)[C@]2(C)[C@H](O)C[C@@]4([H])[C@@]3([H])CC[C@@]5([H])[C@]4(C)CC[C@H](OCC(NCCCCCC(NCC#CC6=CN([C@H]7CC[C@@H](COP(O)(OP(O)(OP(O)(O)=O)=O)=O)O7)C(NC6=O)=O)=O)=O)C5)=C1 |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
