EZ Cap™ Human p53 mRNA
p53 is one of the most studied transcription factors. p53 can activate its downstream genes in response to carcinogenic signals, such as apoptosis promoting protein BAX, p53 upregulates apoptosis regulator PUMA, etc. p53 also acts as a cell cycle checkpoint protector to induce cell cycle arrest and participate in DNA replication and repair to protect genomic integrity.
EZ Cap™ Human p53 mRNA is provided at a concentration of ~1 mg/ml with Cap1 structure. There are currently two ways to cap mRNA: One is co-transcription method, by adding Cap analogues into the transcription process. The other is enzymatic Capping. After transcription, Cap0 capping is performed by Vaccinia virus Capping Enzyme (VCE), GTP and S-adenosylmethionine (SAM). The Cap0 is then generated into the Cap1 through 2´-O-Methyltransferase and SAM. Cap1 Capping can also be performed by adding VCE, 2´-O-Methyltransferase, GTP and SAM in a one-step process. Cap 1 structure is more ideal for mammalian systems and possess higher transcription efficiency than Cap 0 structure. The addition of poly (A) tail increases the stability and lifetime of the mRNA in vitro and in vivo. Poly (A) tail also plays an important role in enhancing the efficiency of translation initiation.
| mRNA Length | 1437 nucleotides |
| Concentration | 1 mg/mL |
| Buffer | 1 mM Sodium Citrate, pH 6.4 |
| Storage | -40°C or below |
| General tips | Please dissolve it on ice and protect from RNase carefully. Avoid repeated freeze/thaw cycles as possible. Don’t vortex. Upon first use, centrifuge the tube softly and aliquot it into several single use portions. Use RNase-free reagents and materials with appropriate RNase-free technique. Don’t add to the media with serum unless mixing with a transfection reagent. |
| Shipping Condition | ship with dry ice |