Hemin Colorimetric Assay Kit
Hemin is an iron-containing porphyrin and comes from the breakdown of hemin-containing proteins such as myoglobin and hemoglobin. Free hemin can be detected in various body fluids (such as csf, saliva and urine) under various pathological states and exists at a very minute concentrations (<1 µM ≈ 650 ng/mL) in cells. Hemin is involved in induction of microsomal hemin oxygenase-1 and inhibition of nonspecific δ-aminolevulinate synthase expression. Hemin also stimulates growth of oral bacteria involved in gingivitis. When found in the urine or feces, it is indicated the possible pathological conditions.
The Hemin Colorimetric Assay Kit provides a highly sensitive, fast and convenient way for detection of hemin levels in various samples based on colorimetric method. The assay utilizes peroxidase activity in the presence of hemin to cause the conversion of a colorless probe to a strongly colored (λ = 570) compound. Trace amounts of Hemin can be quantitated in the 5-160 pg (10-250 fmol) range.
| Kit components | Hemin Assay Buffer Hemin Probe (in anhydrous DMSO) Enzyme Mix Hemin Substrate Hemin Standard (1 nmol) |
| Detection method | Absorbance (570 nm). |
| Sample type | Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth media and food products. |
| Applications | Trace amounts of Hemin can be quantitated in the 5-160 pg (10-250 fmol) range. |
| Features | Fast and convenient. Simple procedure; takes ~ 30 minutes to complete the assay. Highly sensitive assays for measuring Heme level in biological samples. |