HyperChrom Heparin HP Agarose
HyperChrom Heparin HP Agarose is an affinity chromatography medium made by covalently coupling heparin to an agarose base. Heparin is a naturally occurring glycosaminoglycan that has affinity with a variety of biomolecules and can also be used as an ion exchange ligand. This chromatography medium can be used to isolate and purify coagulation factors, antithrombin III, growth factors, interferon, lipoprotein lipase, and enzymes for nucleic acid and steroid receptors.
Compared to HyperChrom Heparin FF Agarose, HyperChrom Heparin HP Agarose has a finer particle size and provides higher resolution.
HyperChrom Heparin HP Agarose chromatography media parameters
|
Dame |
Description |
|
Chromatography media type |
Heparin affinity chromatography mediator |
|
Ligation |
heparin |
|
Scaffolding |
Highly cross-linked agarose |
|
Average particle size |
34 μm |
|
Ligand density |
~10 mg/mL chromatography medium |
|
Flow rates are recommended |
90-150 cm/h |
|
Maximum flow rate |
200 cm/h |
|
Withstand pressure |
0.3 MPa |
|
Use temperature |
4-30℃ |
|
pH stability * |
4-12 |
|
Chemical resistance |
Common aqueous solution, 4 M NaCl, 0.1 M NaOH, 0.05 M sodium acetate (pH4), 6 M guanidine hydrochloride, 8 M urea, 70% ethanol** |
|
* After 7 days of storage of chromatography medium at 40 °C and pH 4-12, its physicochemical properties and functions did not change significantly. **70% is v/v, volume ratio. |
|
| Components | PC2009-25 mL | PC2009-100 mL |
| HyperChrom Heparin HP Agarose | 25 mL | 100 mL |
Store the components at 4℃ for 5 years. | ||