HyperChrom CM HP Agarose
HyperChrom CM HP Agarose is a weak cation exchange chromatography medium that separates molecules using differences in charge properties and amounts under specific conditions. It was found that when sodium hydroxide solution was continuously added dropwise to the chromatography medium, measuring the pH of the supernatant found that it could maintain a fairly wide range of pH stability. It is precisely because of this property that HyperChrom CM HP Agarose chromatography media exhibits an unexpected separation and purification effect. The chromatography medium can be well used for the isolation and purification of a variety of biomolecules, such as: recombinant proteins, antibodies, nucleic acids, viruses and virus-like particles, polysaccharides, etc.
HyperChrom CM HP Agarose has excellent scale-up performance: (1) Highly crosslinked agarose base frame with excellent rigidity, so it can achieve high process flow rates and improve process efficiency at low back pressure. (2) Through chemical modification, it has excellent chemical compatibility and is resistant to CIP cleaning such as sodium hydroxide.
HyperChrom CM HP Agarose chromatography media parameters
|
Name |
Description |
|
Chromatography media type |
Weak cation exchange |
|
Ligation |
-O-CH2COO- |
|
Scaffolding |
Highly cross-linked agarose |
|
Average particle size |
34 μm |
|
Ion load |
0.06-0.08 mmol H+ /mL chromatography medium |
|
Dynamic load |
> 50 mg lysozyme/mL chromatography medium* |
|
Flow rates are recommended |
90-150 cm/h |
|
Maximum flow rate |
200 cm/h |
|
Withstand pressure |
0.3 MPa |
|
Use temperature |
4-30℃ |
|
pH stability ** |
2-14 |
|
Solvent resistant |
Common aqueous solution, 30% isopropanol***, 75% ethanol***, 1 M NaOH, 1 M acetic acid, 6 M guanidine hydrochloride, 8 M urea |
|
Intolerant to solvents |
Oxidant, cationic detergent |
|
* Dynamic load measurement conditions: column loading height: 10cm, test flow rate 300 cm/h; Test buffer: 0.1 M citrate solution, pH 3.0; test sample: 4 mg/mL lysozyme sample, when the penetration of lysozyme reaches 10%, the lysozyme sample volume per unit volume (mL) (mg). **After 7 days in the environment of chromatography at 40oC and pH 2-14, the physical and chemical properties and functions of the chromatography medium did not change significantly. ***30% and 75% are v/v, volume ratio. |
|
| Components | PC2017-25 mL | PC2017-100 mL |
| HyperChrom CM HP Agarose | 25 mL | 100 mL |
Store the components at 4℃ for 5 years. | ||