HyperChrom DEAE FF Agarose
HyperChrom DEAE FF Agarose is a weak anion exchange chromatography medium that separates molecules by taking advantage of differences in charge properties and how much they are under specific conditions. It was found that when sodium hydroxide solution was continuously added dropwise to the chromatography medium, measuring the pH of the supernatant found that it could maintain a fairly wide range of pH stability. It is precisely because of this characteristic that HyperChrom DEAE FF Agarose chromatography media exhibits its unexpected separation and purification effect. The chromatography medium can be well used for the isolation and purification of a variety of biomolecules, such as: recombinant proteins, antibodies, nucleic acids, viruses and virus-like particles, polysaccharides, etc.
HyperChrom DEAE FF Agarose has excellent scale-up performance: (1) Highly crosslinked agarose base with excellent rigidity, so it can achieve high process flow rates and improve process efficiency at low back pressure. (2) Through chemical modification, it has excellent chemical compatibility and is resistant to CIP cleaning such as sodium hydroxide.
HyperChrom DEAE FF Agarose chromatography media parameters
|
Name |
Description |
|
Chromatography media type |
Weak anion exchange |
|
Ligation |
-O-CH2CH2–N ⁺ (C2H5)3H |
|
Scaffolding |
Highly cross-linked agarose |
|
Average particle size |
90 μm |
|
Ion load |
0.11-0.16 mmolCl-/mL chromatography medium |
|
Dynamic load |
> 90 mg ovalbumin/mL chromatography medium* |
|
Flow rates are recommended |
300-600 cm/h |
|
Maximum flow rate |
700 cm/h |
|
Withstand pressure |
0.3 MPa |
|
Use temperature |
4-30℃ |
|
pH stability ** |
2-14 |
|
Solvent resistant |
Common aqueous solution, 30% isopropanol***, 75% ethanol***, 1 M NaOH, 1 M acetic acid, 6 M guanidine hydrochloride, 8 M urea |
|
Intolerant to solvents |
Oxidant, anionic detergent |
|
* Dynamic load measurement conditions: column loading height: 10cm, test flow rate 300 cm/h; Test buffer: 0.05 M Tris-HCl solution, pH 8.0; test sample: 6 mg/ml ovalbumin sample, ovalbumin loading volume per unit volume (mL) (mg) when the penetration of ovalbumin reaches 10%. **After 7 days in the environment of chromatography at 40℃ and pH 2-14, the physical and chemical properties and functions of the chromatography medium did not change significantly. ***30% and 75% are v/v, volume ratio. |
|
| Components | PC2012-25 mL | PC2012-100 mL |
| HyperChrom DEAE FF Agarose | 25 mL | 100 mL |
Store the components at 4℃ for 5 years. | ||