HyperChrom DEAE HP Agarose
HyperChrom DEAE HP Agarose is a weak anion exchange chromatography medium that separates molecules using differences in charge properties and how much they are under specific conditions. It was found that when sodium hydroxide solution was continuously added dropwise to the chromatography medium, measuring the pH of the supernatant found that it could maintain a fairly wide range of pH stability. It is precisely because of this property that HyperChrom DEAE HP Agarose chromatography media exhibits unexpected separation and purification effects. The chromatography medium can be well used for the isolation and purification of a variety of biomolecules, such as: recombinant proteins, antibodies, nucleic acids, viruses and virus-like particles, polysaccharides, etc.
HyperChrom DEAE HP Agarose has excellent scale-up performance: (1) Highly crosslinked agarose base frame with excellent rigidity, so it can achieve high process flow rates and improve process efficiency at low back pressure. (2) Through chemical modification, it has excellent chemical compatibility and is resistant to CIP cleaning such as sodium hydroxide. (3) Fine particle size design to improve resolution.
HyperChrom DEAE HP Agarose chromatography media parameters
|
Name |
Description |
|
Chromatography media type |
Weak anion exchange |
|
Ligation |
-O-CH2CH2–N⁺ (C2H5)3H |
|
Scaffolding |
Highly cross-linked agarose |
|
Average particle size |
34 μm |
|
Ion load |
0.08-0.13 mmol Cl - /mL chromatography medium |
|
Dynamic load |
>90 mg ovalbumin/mL chromatography medium* |
|
Flow rates are recommended |
90-150 cm/h |
|
Maximum flow rate |
200 cm/h |
|
Withstand pressure |
0.3 MPa |
|
Use temperature |
4-30℃ |
|
pH stability ** |
2-14 |
|
Solvent resistant |
Common aqueous solution, 30% isopropanol***, 75% ethanol***, 1 M NaOH, 1 M acetic acid, 6 M guanidine hydrochloride, 8 M urea |
|
Intolerant to solvents |
Oxidant, anionic detergent |
|
* Dynamic load measurement conditions: column loading height: 10cm, test flow rate 150 cm/h; Test buffer: 0.05 M Tris-HCl solution, pH 8.0; test sample: 6 mg/ml ovalbumin sample, ovalbumin loading volume per volume of media (mL) (mg) when ovalbumin penetration reaches 10%. **After 7 days in the environment of chromatography at 40 °C and pH 2-14, the physical and chemical properties and functions of the chromatography medium did not change significantly. ***30% and 75% are v/v, volume ratio. |
|
| Components | PC2013-25 mL | PC2013-100 mL |
| HyperChrom DEAE HP Agarose | 25 mL | 100 mL |
Store the components at 4℃ for 5 years. | ||