HyperChrom Phenyl 6FF (HS) Agarose
HyperChrom Phenyl 6FF (HS) Agoros is a class of hydrophobic interaction chromatography media that takes advantage of differences in the hydrophobic properties and the amount of different molecules under specific conditions. This chromatography medium has a high degree of ligand substitution and is mainly used for the isolation and purification of a variety of biomolecules, such as recombinant proteins, antibodies, viral vaccines, etc.
The HyperChrom Phenyl 6FF (HS) Agoros series offers excellent scale-up performance: (1) Highly crosslinked agarose base with excellent rigidity, resulting in high process flow rates and improved process efficiency at low backpressure. (2) Hydrophilic base frame to minimize the influence on the hydrophobic role of the ligand. (3) Through chemical modification, it has excellent chemical compatibility and is resistant to CIP cleaning such as sodium hydroxide.
HyperChrom Phenyl 6FF (HS) Agarose chromatography media parameters
|
Name |
Description |
|
Chromatography media type |
Hydrophobic interactions |
|
Ligation |
R-O-CH2-CH(OH)-CH2-O-C6H5 |
|
Scaffolding |
Highly cross-linked agarose |
|
Average particle size |
90 μm |
|
Ligand density |
45 μmol phenyl group/mL chromatography medium (HS) |
|
Flow rates are recommended |
150-300 cm/h |
|
Maximum flow rate |
600 cm/h |
|
Withstand pressure |
0.3 MPa |
|
Use temperature |
4-30℃ |
|
pH stability* |
2-14 |
|
Solvent resistant |
Common aqueous solution, 3 M ammonium sulfate, 30% isopropanol**, 75% ethanol**, 10% ethylene glycol**, 1 M NaOH, 1 M acetic acid, 6 M guanidine hydrochloride, 8 M urea |
|
Intolerant to solvents |
Oxidant |
|
* After 7 days of storage of chromatography medium at 40°C and pH 2-14 environment, its physicochemical properties and functions did not change significantly. **30%, 75% and 10% are v/v, volume ratio. |
|
| Components | PC2018-25 mL | PC2018-100 mL |
| HyperChrom Phenyl 6FF (HS) Agarose | 25 mL | 100 mL |
Store the components at 4℃ for 5 years. | ||