HyperChrom Q FF Agarose
HyperChrom Q FF Agarose is a strong anion exchange chromatography medium that separates molecules using differences in charge properties and amounts under specific conditions. The chromatography medium can be well used for the isolation and purification of a variety of biomolecules, such as: recombinant proteins, antibodies, nucleic acids, viruses and virus-like particles, polysaccharides, etc.
HyperChrom Q FF Agarose has excellent scale-up performance: (1) Highly crosslinked agarose base frame with excellent rigidity, so it can achieve high process flow rates and improve process efficiency at low back pressure. (2) Through chemical modification, it has excellent chemical compatibility and is resistant to CIP cleaning such as sodium hydroxide.
HyperChrom Q FF Agarose chromatography media parameters
|
Name |
Description |
|
Chromatography media type |
Strong anion exchange |
|
Ligation |
-N+(CH3)3 (quaternary ammonium). |
|
Scaffolding |
Highly cross-linked agarose |
|
Average particle size |
90 μm |
|
Ion load |
0.18-0.25 mMol Cl - /mL chromatography medium |
|
Dynamic load |
> 90 mg BSA/mL chromatography medium* |
|
Flow rates are recommended |
300-600 cm/h |
|
Maximum flow rate |
700 cm/h |
|
Withstand pressure |
0.3 MPa |
|
Use temperature |
4-30℃ |
|
pH stability ** |
2-14 |
|
Solvent resistant |
Common aqueous solution, 30% isopropanol***, 75% ethanol***, 1 M NaOH, 1 M acetic acid, 6 M guanidine hydrochloride, 8 M urea |
|
Intolerant to solvents |
Oxidant, anionic detergent |
|
* Dynamic load measurement conditions: column loading height: 10cm, test flow rate 300 cm/h; Test buffer: 0.05 M Tris-HCl solution, pH 8.0; test sample: 6 mg/ml BSA sample, BSA loading volume per unit volume (mL) (mg) when the penetration of BSA reaches 10%. ** After 7 days in the environment of chromatography at 40oC and pH 2-14, the physical and chemical properties and functions of the chromatography medium did not change significantly. *** 30% and 75% are v/v, volume ratio. |
|
| Components | PC2010-25 mL | PC2010-100 mL |
| HyperChrom Q FF Agarose | 25 mL | 100 mL |
Store the components at 4℃ for 5 years. | ||