HyperScribe™ T3 High Yield RNA Synthesis Kit
HyperScribe™ T3 High Yield RNA Synthesis Kit performs in vitro RNA transcription experiment in a relatively short time using T3 RNA polymerase. The kit is designed for high yields of RNA transcripts, highly specific radiolabeled RNA probes and to obtain capped, dye-labeled or biotinylated RNA incorporated modified nucleotides.
RNA synthesized using this kit covers a wide range of applications, such as in vitro translation, antisense RNA and RNAi experiments, RNA vaccines, RNA structure and function studies, ribozyme biochemistry, RNase protein experiments and probe-based hybridization blots.
The kit contains sufficient reagents to carry out 25/50/100 reactions, 20 μL each time. ≥50 μg of RNA can be generated with 1 μg of control template per standard reaction.
| Components | 25 rxns | 50 rxns | 100 rxns |
| T3 RNA Polymerase Mix | 50 μL | 100 μL | 200 μL |
| 10 × Reaction Buffer | 50 μL | 100 μL | 200 μL |
| ATP (100 mM) | 25 μL | 50 μL | 100 μL |
| GTP (100 mM) | 25 μL | 50 μL | 100 μL |
| UTP (100 mM) | 25 μL | 50 μL | 100 μL |
| CTP (100 mM) | 25 μL | 50 μL | 100 μL |
| Control Template (0.5 μg/μL) | 5 μL | 10 μL | 20 μL |
| RNase-free H2O | 0.5 mL | 1 mL | 2 x 1 mL |
| DNase I (RNase-free) (2 U/µl) | 25 μL | 50 μL | 100 μL |
Store all the kit components at -20°C. | |||