HyperScribe™ T7 High Yield Fluorescein RNA Labeling Kit Plus
HyperScribeTM T7 High Yield Fluorescein RNA Labeling Kit Plus is designed to generate randomly fluorescein-modified RNA probes by in vitro transcription. Such probes are ideally suited for in situ hybridization and Northern blot hybridization experiments. The principle of labeling is similar to the basic labeling principle of a mixture of fluorescein RNA labels. Fluorescein-12-UTP is efficiently incorporated into RNA using the optimized reaction buffer and T7 RNA polymerase mixture in place of its natural counterpart UTP. An appropriate Fluorescein-12-UTP substitution typically achieves an optimal balance between reaction and labeling efficiency. The resulting fluorescein-modified RNA probe can then be detected by fluorescence spectroscopy.
This kit adjusts the composition and reaction based on K1060 to achieve higher RNA yields for standard 20 μL reaction systems.
| Components | 25 rxn |
| T7 RNA Polymerase Mix | 50 μL |
| 10 × Reaction Buffer | 50 μL |
| ATP (100 mM) | 30 μL |
| UTP (100 mM) | 19 μL |
| CTP (100 mM) | 25 μL |
| GTP (100 mM) | 25 μL |
| Fluorescein-12-UTP (10 mM) | 62.5 μL |
| Control Template (0.5 μg/μL) | 5 μL |
| RNase-free H2O | 0.5 mL |
Store the components at -20°C. | |