HyperScribe™ T7 High Yield RNA Synthesis Kit (N1-Methylpseudo-UTP)
HyperScribeTM T7 High Yield RNA Synthesis Kit performs in vitro RNA transcription experiment in a relatively short time using T7 RNA polymerase. The addition of N1-Methylpseudo-UTP(m1Ψ) (Cat. No. B8049) can reduce host cell immune response. The kit is designed for high yields of RNA transcripts, highly specific radiolabeled RNA probes and to obtain capped, dye-labeled or biotinylated RNA incorporated modified nucleotides. RNA synthesized using this kit covers a wide range of applications, such as in vitro translation, antisense RNA and RNAi experiments, RNA vaccines, RNA structure and function studies, ribozyme biochemistry, RNase protein experiments and probe-based hybridization blots.
The kit contains sufficient reagents to carry out 25/50/100 reactions, 20 μL each time. Up to 180 μg of RNA can be generated with 1 μg of control template per standard reaction.
|
Components |
25 rxns |
50 rxns |
100 rxns |
|
T7 RNA Polymerase Mix |
50 μL |
100 μL |
200 μL |
|
10 × Reaction Buffer |
50 μL |
100 μL |
200 μL |
|
ATP (100 mM) |
25 μL |
50 μL |
100 μL |
|
GTP (100 mM) |
25 μL |
50 μL |
100 μL |
|
N1-Methylpseudo-UTP (100 mM) |
25 μL |
50 μL |
100 μL |
|
CTP (100 mM) |
25 μL |
50 μL |
100 μL |
|
Control Template (0.5 μg/μL) |
5 μL |
10 μL |
20 μL |
|
RNase-free H2O |
0.5 mL |
1 mL |
2 x 1 mL |
|
Store the components at -20°C. |
|||