HyperScribe™ T7 High Yield RNA Synthesis Kit Plus
HyperScribe™ T7 High Yield RNA Synthesis Kit Plus performs in vitro RNA transcription experiment in a relatively short time using T7 RNA polymerase. The kit is designed for high yields of RNA transcripts, highly specific radiolabeled RNA probes and to obtain capped, dye-labeled or biotinylated RNA incorporated modified nucleotides.
RNA synthesized using this kit covers a wide range of applications, such as in vitro translation, antisense RNA and RNAi experiments, RNA vaccines, RNA structure and function studies, ribozyme biochemistry, RNase protein experiments and probe-based hybridization blots.
The kit contains sufficient reagents to carry out 25/50/100 reactions, 20 μL each time. Up to 100 μg of RNA can be generated with 1 μg of control template per standard reaction. Each 25/50/100 reactions kit can produce up to 4.5 mg/9 mg/18 mg RNA.
Figure 1: Transcription using T7 RNA Polymerase
Figure 2: PCR primer design for T7 RNA Polymerase template
Quality Control & DataSheet
- View current batch:
| Components | 25 rxns | 50 rxns | 100 rxns |
| T7 RNA Polymerase Mix | 50 μL | 100 μL | 200 μL |
| 10X Reaction Buffer | 50 μL | 100 μL | 200 μL |
| ATP (100 mM) | 25 μL | 50 μL | 100 μL |
| GTP (100 mM) | 25 μL | 50 μL | 100 μL |
| UTP (100 mM) | 25 μL | 50 μL | 100 μL |
| CTP (100 mM) | 25 μL | 50 μL | 100 μL |
| Control Template(0.5 μg/μL) | 5 μL | 10 μL | 20 μL |
| RNase-free H2O | 0.5 mL | 1 mL | 2 x 1 mL |
Store all the kit components at -20°C. | |||