HyperScript™ First-Strand cDNA Synthesis SuperMix (with gDNA wiper)
HyperScript™ First-Strand cDNA Synthesis SuperMix (with gDNA wiper) is based on HyperScript™ Reverse Transcriptase (Cat. No. K1071) to synthesize first-strand cDNA from Total RNA or Poly(A)+ RNA. HyperScript™ Reverse Transcriptase is a new enzyme obtained through genetic engineering based on M-MLV (RNase H-) Reverse Transcriptase. In comparison, HyperScript™ Reverse Transcriptase reduces RNase H activity and increases thermal stability. HyperScript™ Reverse Transcriptase can withstand higher reaction temperatures and is suitable for reverse transcription of RNA templates with complex secondary structures. In addition, HyperScript™ Reverse Transcriptase enhances the affinity to the template, suitable for the reverse transcription of a small number of templates and low copy genes and can generate cDNA up to 12.3 kb.
HyperScript™ First-Strand cDNA Synthesis SuperMix (with gDNA wiper) contains all components except primers required for the synthesis of the first strand cDNA and provides two cDNA synthesis primers, Random Primers and Oligo (dT)23VN. The 4× gDNA wiper included in the product quickly and thoroughly removes potential genomic interference from the system before reversing transcription. You can choose Random Primers, Oligo (dT)23VN or gene-specific primers as reverse transcription primers according to the needs of the experiment. The synthesized first-strand cDNA products can be used in subsequent experiments such as PCR amplification and qPCR reaction.
| Components | 50 rxns (20 μL reaction) | 100 rxns (20 μL reaction) |
| RNase Free ddH2O | 1 mL | 1 mL x 2 |
| 5X SuperMix | 200 μL | 400 μL |
| 4X gDNA wiper mix | 190 μL | 380 μL |
| Random Primers (50 μM) | 50 μL | 100 μL |
| Oligo (dT) 23VN (50 μM) | 50 μL | 100 μL |
| 5X control Mix | 20 μL | 40 μL |
Store the components at -20°C. | ||
SuperMix contains all components except primers required for the synthesis of the first strand cDNA, easy to use.
Efficient HyperScript™ Reverse Transcriptase for RNA templates containing secondary structures
Synthesis of first-strand cDNA up to 12.3 kb
Detects template starting amounts as low as 1 pg
Oligo (dT)23VN primers have stronger template anchoring ability and higher reverse transcription efficiency than Oligo (dT)18
Synthesized first-strand cDNA can be used for experiments such as PCR amplification and qPCR reaction