HyperScript™ III First-Strand cDNA Synthesis SuperMix (with gDNA wiper)
HyperScript™ III Reverse Transcriptase is a third-generation reverse transcriptase based on M-MLV Reverse Transcriptase that has been genetically engineered. Compared to wild-type, HyperScript™ III Reverse Transcriptase generates a reduced RNase H activity and improves thermal stability and fidelity. In addition, it has characteristics of higher cDNA synthesis yield and longer length, higher reverse transcription efficiency for high-GC content RNA. HyperScript™ III Reverse Transcriptase has an enhanced affinity for templates and is suitable for reverse transcription of small amounts of template and low-copy genes, you can use PCR to amplify cDNA up to 12.3 kb.
HyperScript™ III First-Strand cDNA Synthesis SuperMix (with gDNA wiper) is a kit based on HyperScript™ III Reverse Transcriptase, which contains all components except primers and templates required for the reaction. The kit supplies Random Primers and Oligo (dT)23VN primer. The inclusion of the 4× gDNA wiper in the product is intended to remove potential genomic DNA contamination before reverse transcription. Random Primers, Oligo (dT)23VN or gene-specific primers can be chosen as reverse transcription primers according to your needs, and the synthesized first-strand cDNA products can be used in subsequent PCR amplification, qPCR reactions, and other experiments.
| Components | 50 rxn (20 μL reaction) | 100 rxn (20 μL reaction) |
| RNase Free ddH2O | 1 mL | 2×1 mL |
| 5×HyperScript™ III SuperMix | 200 μL | 400 μL |
| 4×gDNA wiper mix | 190 μL | 380 μL |
| Random Primers (50 μM) | 50 μL | 100 μL |
| Oligo (dT)23VN (50 μM) | 50 μL | 100 μL |
| 5×HyperScript™ III control Mix | 20 μL | 40 μL |
Store the components at -20℃ for 2 years. | ||