HyperScript™ IV First-Strand cDNA Synthesis SuperMix (with gDNA wiper)
HyperScript™ IV Reverse Transcriptase is a genetically engineered fourth-generation reverse transcriptase based on M-MLV and provides superior robustness and reliability in RT reactions. The enzyme has significant improvements in inhibitor resistance, processivity, and reaction speed while retaining all the advantages of HyperScript™ III Reverse Transcriptase, including thermostability, highly efficient full-length cDNA synthesis, and reduced RNase H activity. The product can still provide reliable, consistent, and rapid cDNA synthesis in the presence of inhibitors (residues from RNA extraction).
The kit contains all components for the synthesize of first-strand cDNA and provides two kinds of primers for cDNA synthesis as Random Primer and Oligo(dT)23VN. The inclusion of the 4× gDNA wiper in the product is intended to remove potential genomic DNA contamination before reverse transcription. Random Primer, Oligo(dT)23VN or gene-specific primer can be chosen as reverse transcription primer according to your needs, and the products can be used in subsequent PCR amplification, qPCR reactions and other experiments.
| Components | 50 rxn (20 μL reaction) | 100 rxn (20 μL reaction) |
| RNase Free ddH2O | 1 ml | 2×1 mL |
| 5×HyperScript™ IV SuperMix | 200 μL | 400 μL |
| 4×gDNA wiper mix | 190 μL | 380 μL |
| Random Primers (50 μM) | 50 μL | 100 μL |
| Oligo (dT)23VN (50 μM) | 50 μL | 100 μL |
| 5×HyperScript™ IV control Mix | 20 μL | 40 μL |
Store the components at -20℃ for 2 years. | ||