HyperTrap AR rProtein A FF Column
HyperChrom AR rProtein A FF Agarose is an affinity chromatography medium for antibody purification, separated by a specific interaction between antigen and antibody. HyperTrap AR rProtein A FF Column Ready-to-use chromatography column with HyperChrom AR rProtein A FF Agarose as the packing is mainly used for the purification of monoclonal and polyclonal antibodies, with the advantages of high flow rate, high load, easy regeneration, low cost and easy amplification.
The main body and inner plug of the pre-installed column tube are made of PP, and the inside and outside are polished, and the sieve plate is made of HDPE material, which has excellent physical properties and chemical resistance, corrosion resistance, anti-aging, long life and good safety. 1) Ready-to-use, convenient and fast. 2) Can be used with syringes, peristaltic pumps or chromatography systems. 3) It can be used in series to increase the sample processing capacity.
1. HyperTrap AR rProtein A FF Column preloaded column parameters
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Pre-assembled column specification/name |
1 mL |
5 mL |
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Dimensions (IDxH) |
7.7 x 25 mm |
16 x 25 mm |
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Withstand pressure |
0.3 MPa |
0.3 MPa |
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Workflow Speed* |
1 mL/min |
1-3 mL/min |
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Operating temperature |
4 - 30°C |
4 - 30°C |
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Storage condition |
Store the components at 4℃ for 5 years. |
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*This flow rate is the recommended common flow rate, the pressure resistance, and the use of different types of chromatography packing in the pre-assembled column will be different, please refer to the product manual of the corresponding chromatographic filler. |
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2. HyperTrap AR rProtein A FF Column chromatography media parameters
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Name |
Description |
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Chromatography media type |
Affinity chromatography media |
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Ligation |
rProtein A (E. coli recombinant expression) |
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Scaffolding |
Highly cross-linked agarose |
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Average particle size |
90 μm |
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Ligand density |
6 mg rProtein A/ml chromatography medium |
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Dynamic load |
~35 mg human IgG/mL chromatography medium* |
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Flow rates are recommended |
150-500 cm/h |
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Maximum flow rate |
700 cm/h |
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Withstand pressure |
0.3 MPa |
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Use temperature |
4-30℃ |
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pH stability ** |
3-10 |
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Chemical resistance |
6 M guanidine hydrochloride, 8 M urea, 2% benzyl alcohol, 75% ethanol, 1 mM NaOH (pH11), 0.1 M sodium citrate/HCl (pH3). |
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** Dynamic load measurement conditions: column loading height: 5 cm, retention time 3 min, test buffer: 0.02 M NaH2PO4 solution, pH 7.0, test sample: human lgG sample, lgG sample per unit media volume (ml) when the penetration of lgG reaches 10%. **After 7 days of storage of chromatography medium at 40°C and pH 3-10, its physicochemical properties and functions did not change significantly. ***30% and 75% are v/v, volume ratio. |
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