HyperTrap Heparin HP Column
HyperChrom Heparin HP Agarose is an affinity chromatography medium made by covalently coupling heparin to an agarose base. Heparin is a naturally occurring glycosaminoglycan that has affinity with a variety of biomolecules and can also be used as an ion exchange ligand. This chromatography medium can be used to isolate and purify coagulation factors, antithrombin III, growth factors, interferon, lipoprotein lipase, and enzymes for nucleic acid and steroid receptors. Compared to HyperChrom Heparin FF Agarose, the HyperChrom Heparin HP Agarose has a finer particle size and provides higher resolution.
HyperTrap Heparin HP Column uses HyperChrom Heparin HP Agarose as a chromatography medium for the isolation and purification of coagulation factors, antithrombin III, growth factors, interferon, lipoprotein lipase, and enzymes for nucleic acid and steroid receptors.
The main body and inner plug of the pre-installed column tube are made of PP, and the inside and outside are polished, and the sieve plate is made of HDPE material, which has excellent physical properties and chemical resistance, corrosion resistance, anti-aging, long life and good safety. 1) Ready-to-use, convenient and fast. 2) Can be used with syringes, peristaltic pumps or chromatography systems. 3) It can be used in series to increase the sample processing capacity.
1. HyperTrap Heparin HP Column preloaded column parameters
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Pre-assembled column specification/name |
1 mL |
5 mL |
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Dimensions (IDxH) |
7.7 x 25 mm |
16 x 25 mm |
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Withstand pressure |
0.3 MPa |
0.3 MPa |
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Workflow Speed* |
1 mL/min |
1-3 mL/min |
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Operating temperature |
4 - 30°C |
4 - 30°C |
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Storage condition |
Store the components at 4℃ for 5 years. |
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*This flow rate is the recommended common flow rate, the pressure resistance, and the use of different types of chromatography packing in the pre-assembled column will be different, please refer to the product manual of the corresponding chromatographic filler. |
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2. HyperTrap Heparin HP Column chromatography media parameters
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Dame |
Description |
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Chromatography media type |
Heparin affinity chromatography mediator |
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Ligation |
heparin |
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Scaffolding |
Highly cross-linked agarose |
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Average particle size |
34 μm |
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Ligand density |
~10 mg/mL chromatography medium |
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Flow rates are recommended |
90-150 cm/h |
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Maximum flow rate |
200 cm/h |
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Withstand pressure |
0.3 MPa |
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Use temperature |
4-30℃ |
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pH stability * |
4-12 |
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Chemical resistance |
Common aqueous solution, 4 M NaCl, 0.1 M NaOH, 0.05 M sodium acetate (pH4), 6 M guanidine hydrochloride, 8 M urea, 70% ethanol** |
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* After 7 days of storage of chromatography medium at 40 °C and pH 4-12, its physicochemical properties and functions did not change significantly. **70% is v/v, volume ratio. |
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