IL-6, rat recombinant
Interleukin-6 (IL-6) is a pleiotropic cytokine produced by activated T cells, monocytes, fibroblasts, endothelial cells, keratinocytes and macrophages, and plays an critical role in host defense by regulating immune and inflammatory responses. IL-6 stimulates B cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins and regulates bone metabolism. Murine IL-6 is inactive on human cells, while both human and murine IL-6 are equally active on murine cells.
The rat IL-6 cDNA encodes a 211 aa protein with a 24 aa signal sequence. Recombinant rat IL-6 is a 21.7 kDa protein containing 187 amino acid residues. IL-6 is an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections and is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha.
- 1. Xiao-yan Zhou, Rong Gao, et al. "Trained Innate Immunity by Repeated Low-Dose Lipopolysaccharide Injections Displays Long-Term Neuroprotective Effects." Mediators Inflamm. 2020 Oct 1;2020:8191079. PMID:33061831
- 2. Zhou H, Sun Y, et al. "Paris saponin VII extracted from trillium tschonoskii suppresses proliferation and induces apoptosis of human colorectal cancer cells." J Ethnopharmacol. 2019 Jul 15;239:111903. PMID:31047966
| Determined by the dose-dependent stimulation of the proliferation of the IL-6 dependent murine 7TD1 cells. | ||||||
| ED50 | < 0.01 ng/ml. | |||||
| Specific activity | > 1*108 units/mg. | |||||
Quality Control & DataSheet
- View current batch:
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Purity: ≥90%, by SDS-PAGE and HPLC.
Endotoxin Level: <0.1 ng/μg
Related Biological Data
Related Biological Data
| Gene ID: | 24498 | Accession #: | P20607 |
| Alternate Names: | IL6, IFN-b2, B cell differentiation factor (BCDF), BSF-2, HPGF, HSF, MGI-2, IL-6, Interleukin HP-1, B-cell hybridoma growth factor. | ||
| Source: | Escherichia Coli. | M.Wt: | 21.7 kDa |
| Appearance: | Lyophilized protein | Storage: | Store at -20°C |
| Formulation: | Lyophilized with no additives. | ||
| Solubility: | Reconstitute in 10 mM HCl to a concentration of 0.1-1.0 µg/µl. This solution can then be diluted into other aqueous buffers and stored at 4℃ for 1 week or -20℃ for future use. | ||
| Shipping Condition: | Blue ice. | Handling: | Centrifuge the vial prior to opening. |
| Usage: | For Research Use Only! Not to be used in humans. | ||