IL-8 (77 a.a.), human recombinant
Interleukin-8 (IL-8) is a proinflammatory CXC chemokine produced by macrophages, epithelial cells. IL-8 is also synthesized by endothelial cells, which store IL-8 in their storage vesicles, the Weibel-Palade bodies. In vivo, IL-8 exists in two forms: a 77 a.a. protein produced by endothelial cells, and the more active 72 a.a. protein produced by monocytes. IL-8 (72 a.a.) has a 5-10 fold higher activity on neutrophil activation compared to IL-8 (77 a.a.). The receptors for IL-8 are the seven-helical G-protein coupled receptors CXCR1 and CXCR2, exclusively expressed on neutrophils. IL-8 serves as a chemical signal that attracts neutrophils at the site of inflammation and thus enhances the antimicrobial actions of defense cells.
The human IL-8 cDNA encodes a 99 aa protein with a 20 aa signal sequence. Recombinant human IL-8 (endothelial-derived) is a 8.9 kDa protein containing 77 amino acid residues.
| Determined by its ability to chemoattract human peripheral blood neutrophils using a concentration range of 25 to 150 ng/ml. |
Quality Control & DataSheet
- View current batch:
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Purity: ≥98%, by SDS-PAGE and HPLC.
Endotoxin Level: <0.1 ng/μg
Related Biological Data
Related Biological Data
| Gene ID: | 3576 | Accession #: | P10145 |
| Alternate Names: | Human IL-8 (77 a.a.), IL-8 (77 a.a.), m-IL-8 (77 a.a.), rh-IL-8 (77 a.a.), recombinant human IL-8, IL-8,IL8, IL 8, m-IL-8, rh-IL-8, recombinant IL-8, IL, interluekin. | ||
| Source: | Escherichia Coli. | M.Wt: | 8.9 kDa |
| Appearance: | Lyophilized protein | Storage: | Store at -20°C |
| Formulation: | Sterile filtered and lyophilized with no additives. | ||
| Solubility: | Reconstitute in H2O to a concentration of 0.1-1.0 µg/µl. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use. | ||
| Shipping Condition: | Gel pack. | Handling: | Centrifuge the vial prior to opening. |
| Usage: | For Research Use Only! Not to be used in humans. | ||