Caspase-4 Colorimetric Assay Kit
Caspase-4 belongs to the caspase-family of cysteine proteases. Caspase-4 is an enzyme that proteolytically cleaves other proteins at an aspartic acid residue. Caspase-4 exists in cells as an inactive proenzyme. It is matured by proteolysis. The active Caspase-4 is a heterotetramer contain two large and two small subunits. The function of Caspase 4 is believed to be an inflammatory caspase, together with Caspase-1, Caspase-5, with a role in the immune system.
Apoptosis in mammalian cells is caused by activation of ICE-family proteases/caspases. The Caspase-4 Fluorometric Assay Kit provides a fast and simple means to assay the activity of caspases that recognize the sequence LEVD. The assay is according to spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate LEVD-pNA. Using a spectrophotometer or a microtiter plate reader at 400 or 405 nm can quantify the pNA light emission. Comparison of the absorbance of pNA from an apoptotic sample with an un-induced control allows determination of the fold increase in LEVD-dependent caspase activity.
| Kit components | Cell Lysis Buffer, 2X Reaction Buffer, LEVD-pNA (4 mM), DTT (1 M), Dilution Buffer |
| Detection method | Absorbance (400 or 405 nm) |
| Sample type | Cell and tissue lysates |
| Features | Fast and convenient; Simple one-step procedure; Takes only 1-2 hours; Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in LEVD-dependent caspase activity. |
| Shipping | Gel pack. |
| Storage Conditions | Store at -20°C. |