Caspase Fluorometric Substrate Set Plus
Caspases (Cysteine-dependent aspartate-directed proteases) are a family of cysteine proteases that play important roles in apoptosis, inflammation and necrosis. Sequential activation of caspases plays a critical role in cell apoptosis. Caspase-1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 are members of caspases family. Caspase-1, 4 and 5 are involved in T-cell maturation. Caspase-2, 8, 9 and 10 are initiator caspases that cleave inactive pro-forms of effector caspases into active caspases. Caspase-3, 6 and 7 are effector caspases that cleave other protein substrates within the cell to trigger the apoptotic process.
Caspase Fluorometric Substrate Set Plus is composed of ready-to-use AFC-labeled substrates for caspase-1, 2, 3, 5, 6, 8 and 9 of caspase family proteases. The kit is used to detect activities of members of caspase family proteases. All substrates are provided in liquid ready-to-use form.
| Features | Contains ready-to-use caspase-1/-2/-3/-5/-6/-8/-9 AFC-labeled substrates and all buffers for performing caspase assay. |
| Shipping | Gel pack. |
| Storage Conditions | Store at -20°C. |
| Kit components | Caspase-1 Substrate, Ac-YVAD-AFC, Caspase-2 Substrate, Ac-VDVAD-AFC, Caspase-3 Substrate, Ac-DEVD-AFC, Caspase-5 Substrate, Ac-WEHD-AFC, Caspase-6 Substrate, Ac-VEID-AFC, Caspase-8 Substrate, Ac-IETD-AFC, Caspase-9 Substrate, Ac-LEHD-AFC, Cell Lysis Buffer, 2X Reaction Buffer, DTT (1 M) |