Ethanol Colorimetric/Fluorometric Assay Kit

Ethanol (C₂H₅OH) is produced by the fermentation of sugars by yeasts and is present in alcoholic beverages. Low doses of alcohol is benefit for circulation while high doses of alcohol consumption may lead to various diseases. Quantitative determination of alcohol is of important significance in drug discovery, basic research, clinical studies and fermentation industry processes.

The Ethanol Colorimetric/Fluorometric Assay Kit provides a sensitive, fast and convenient way for detection of ethanol concentration in various biological samples such as plasma, serum, other body fluids, growth media, foods and beverages based on colorimetric and fluorometric method. Alcohol oxidase oxidizes ethanol to produce H₂O₂ which reacts with Ethanol Probe to yield fluorescence (Ex/Em = 535/587 nm) and color (λmax = 570 nm). The kit can detect 0.1-10 ppm alcohol (~10-800 nM).