Phosphate Assay Kit (Fluorometric)

Phosphate is an important inorganic ion in biological systems and has many functions. Phosphate can turn on or off enzyme activity through the mediation of the various phosphatases and protein kinases in biological systems. Phosphate also plays an important role in mineralization processes and is a major stimulus of algal blooms mainly found in bodies of fresh water, because of run-off from areas of high fertilizer use.

 The Phosphate Assay Kit (Fluorometric) provides a highly sensitive, easy and fast way for detection of phosphate (Pi) over a wide range of concentrations in various samples based on fluorometric method. In the presence of a proprietary enzyme, inorganic phosphate reacts with sucrose to produce glucose-1-phosphate, which is specifically oxidized to yield a product that reacts with PicoProbe™ to produce fluorescence (Ex/Em = 535/587 nm). The kit can be used to detect phosphate produced through reactions involving GTPases, ATPases, acid and alkaline phosphatases, phosphorylase, 5’-nucleotidase, protein phosphatases, etc. from a variety of samples. In addition, the assay is not affected by the presence of glucose in samples. This kit can detect phosphate concentrations between 2 µM and 10 µM, with a lower limit of approximately 100 pmol.