Phosphatidylcholine Colorimetric/Fluorometric Assay Kit

Phosphatidylcholine (PC) is a class of phospholipid that incorporates choline as the lipid headgroup. PC is a major component of pulmonary surfactant and biological membranes. PC can transport by phosphatidylcholine transfer protein (PCTP) between membranes within the cell. PC is also involved in membrane-mediated cell signaling through the second messenger phosphatidic acid.

The Phosphatidylcholine Colorimetric/Fluorometric Assay Kit provides a sensitive, simple and convenient way for detection of phosphatidylcholine in various biological fluids based on colorimetric and fluorometric method. The assay is based on an enzyme-coupled assay in which PC is hydrolyzed to release choline, which is then oxidized resulting in development of the OxiRed probe to yield fluorescence (Ex/Em 535 nm 587 nm) and absorbance (570 nm). The kit can measure PC in the range of 0.1 to 10 nmol per sample. PC in serum is about 0.2-2.5 mM (~50-200 mg/dL).