Beta-Secretase Activity Fluorometric Assay Kit

β-Secretase is an aspartic-acid protease that plays an important role in Alzheimer’s disease and has been an excellent target for the treatment of Alzheimer’s disease based on anti-amyloid therapy.

 The Beta-Secretase Activity Fluorometric Assay Kit provides a highly sensitive and convenient way for detection of β-secretase activity in various biological and purified samples based on fluorescence method. The assay utilizes a β-secretase-specific peptide substrate conjugated to two molecules EDANS and DABCYL. In the uncleaved form, the fluorescent emissions from EDANS are quenched by the DABCYL moiety because of the physical proximity. Upon cleavage by β-secretase, EDANS is physically separated with DABCYL, which allowing for the release of a fluorescent signal. The activity of β-secretase in samples is proportionally related to the level of fluorescence intensity.