Lambda Exonuclease
Catalog No.
K1097
5’→3’ DNA-specific exonuclease
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Lambda Exonuclease is a DNA-specific exonuclease that progressively degrades double-stranded DNA from a 5' end in the 5' →3' direction. The optimal substrate is 5' phosphorylated double-stranded DNA, which also slowly degrades single-stranded DNA and non-phosphorylated substrates. The enzyme cannot begin digestion from the incision or notch of dna. The product can be used for 1) converting double-stranded DNA into single-stranded DNA; 2) DNA single-strand conformation polymorphism (SSCP) analysis; 3) Degradation of linearized plasmids, retention of supercoil plasmids; 4) Roll ring amplification, etc.
| Component | 1000 U | 5000 U | 10000 U |
| Lambda Exonuclease | 0.2 ml | 1 ml | 2 ml |
| 10X Lambda Exonuclease Reaction Buffer | 0.1 ml | 0.5 ml | 1 ml |
Long-term storage: -20 °C | |||