Live-Dead Cell Staining Kit I (Calcein AM/PI)
Live-Dead Cell Staining Kit I (Calcein AM/PI) is used for simultaneous fluorescence staining of live and dead mammalian cells. This kit contains two probes, Calcein AM and propidium iodide (PI). Calcein AM is a live-cell probe, which is highly lipophilic and cell permeable. Calcein AM itself is no-fluorescent, but it is hydrolyzed into green-fluorescent Calcein by esterase in the viable cells. On the other hand, PI is a dead cell probe that can only pass through damaged cell membranes and stain the nucleus red. The fluorescence intensity of these two probes can reflect cell viability and toxicity.
The kit is sensitive, quick and easy to use. However, Calcein AM does not penetrate the cell walls of bacteria or fungi, so this kit is not suited for bacteria or fungi.
Figure 1: The effect of this kit for the detection of live/dead cells
| Components | K2247-100T | K2247-500T | K2247-2500T |
| Calcein AM (1000x) | 10 μL | 50 μL | 250 μL |
| PI (1000x) | 10 μL | 50 μL | 250 μL |
| Staining Buffer | 10 mL | 50 mL | 250 mL |
Store the kit at -20°C, stable for 1 year. The Calcein AM (1000x) and PI (1000x) should be stored away from light and moisture, avoiding repeated freeze/thaw cycles. | |||